Elucidating protein function

13-Dec-2017 04:56

A 'protein sequence ontology' to facilitate comparison of the annotations has also been developed (G Reeves, personal communication).Automated strategy for assigning function to proteins.World Health Organization estimates that one in eight person die from air pollution.The main objective of our study is to analyze the feasibility of genetically engineered plants to remove polluting volatile compounds from air.To achieve this goal we genetically transformed Arabidopsis thaliana with an ACS (Aminocyclopropane-1-carboxylic acid synthase) gene.Using quantitative real-time PCR (q PCR), we previously confirmed that the ACS gene was upregulated in poplar (Populus trichocarpa) plants when exposed to environmental pollutants including ozone and methyl vinyl ketone.The investigation of sequence-function and structure-function relationships has therefore become a fundamental necessity.Understanding these relationships will be crucial for moving from an inventory of protein parts to a more profound understanding of the molecular machinery of organisms at a systems level. DAS allows different laboratories to 'combine' their annotations, produced by both experimental and computational approaches, to generate a 'composite' annotation at all levels.

As opposed to traditional structural biology, the determination of a protein structure through a structural genomics effort often (but not always) comes before anything is known regarding the protein function.

Another common and stable form of chromium in the environment is hexavalent chromium (Cr).